TABLE 1.

Metabolism of (+)- and (−)-benzylsuccinate by permeabilized strain T cells^a

Benzylsuccinate isomer added	Amt detected after incubation (nmol)
	Benzylsuccinate	E-phenylitaconate	Benzoate
(+)	<DLb	37	3
(−)	50	0.4	0.9
None	<DL	0.3	0.7

^aThe anaerobic assay mixtures (total volume, 1 ml; incubation time, 1 h) were amended with (+)- or (−)-benzylsuccinate (100 nmol, except in the control), succinyl-CoA (0.35 mM), nitrate (2 mM), titanium(III) chloride (0.2 mM), and ∼3 mg of protein from permeabilized, toluene-grown strain T cells. Analytical recovery of benzylsuccinate in these experiments was approximately 51% ± 3.9% (mean ± standard deviation) based on benzylsuccinate-amended control experiments without permeabilized cells (data not shown) and the (−)-benzylsuccinate-amended assays represented in the table. 

^b<DL, less than the detection limit of approximately 0.2 nmol.