TABLE 2.
Molecular weight distributions of benzylsuccinate molecules in an unlabeled benzylsuccinate standard and formed during an in vitro assay
| Benzylsuccinate source | Relative distributiona (%) of benzylsuccinates with a molecular wt of:
|
||||
|---|---|---|---|---|---|
| 236 | 237 | 238 | 239 | 240 | |
| Assay (equimolar toluene and toluene-d3)b | 100 | 16 | ≤1 | 70 | 9.0 |
| Unlabeled benzylsuccinate (dimethyl ester) standard | 100 | 15 | 2 | <DLc | <DL |
a
Areas of dimethyl benzylsuccinate peaks integrated with quantification ions of m/z 236, 237, 238, 239, and 240 normalized to the area for m/z 236.
b
The total amount of benzylsuccinate formed in this assay amended with ∼400 nmol of toluene was 250 to 300 nmol. Assay conditions are given in the text.
c
<DL, less than the detection limit of approximately 1.