TABLE 1.
Bacteria, plasmid vectors, and pas constructs used in this study
| Strain, plasmid, or construct | Genotype or descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| JM105 | thi rpsL endA sbcB15 hspR4 Δ(lac-proAB) [F′ traD36 proAB lacIqΔM15] | 20 |
| JM107 | thi endA gyrA96 hsdR17 supE44 relA1 Δ(lac-proAB) [F′ traD36 proAB lacIqΔM15] | 20 |
| JM109 | recA1 thi endA gyrA96 hsdR17 supE44 relA1 Δ(lac-proAB) [F′ traD36 proAB lacIqΔM15] | 20 |
| 71/18 | thi supE Δ(lac-proAB) [F′ traD36 proAB lacIqΔM15] | 20 |
| CSH50 | rpsL Δ(lac-pro) | 12 |
| CSH50-Iq | rpsL Δ(lac-pro) [F′ traD36 proAB lacIqΔM15] | This work |
| SG22025 | Δlac rcsA166::mini-kan parent of SG22093 and SG22095 | S. Gottesman (11) |
| SG22093 | Δlac rcsA166::mini-kan clpP1::cat | S. Gottesman (11) |
| SG22095 | Δlac rcsA166::mini-kan lon-146::mini-Tn10 | S. Gottesman (11) |
| Plasmids | ||
| pACYC184 | p15a replicon, Cmr Tcr | 3 |
| pKK223-3 | ColE1 replicon, Aprtac | 2 |
| pOU82 | R1 replicon, AprlacZYA | 6 |
| pKG399 | pSC101 replicon, Tcr (see Fig. 3) | 9 |
| pKGCm | pACYC184 replicon, Cmr (see Fig. 3) | This work |
| pas-containing constructs | ||
| pTac-pasB | pKK223-3 replicon, Apr, pas region 1518–1816b | 17 |
| pTac-pasAB | pKK223-3 replicon, Aprpas region 1316–1816 | 17 |
| pTac-pasABC | pKK223-3 replicon, Aprpas region 1316–2028 | 17 |
| pOU-pasABC | pOU82 replicon (R1), Apr, pas region 1158–2027 | 17 |
| pOU-pasABC*c | pOU82 replicon (R1), Apr, pas region 1158–2027 | 17 |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance; tac, trp-lac hybrid promoter.
b
The pas region numbers are the nucleotide positions relative to those of the ClaI-PstI fragment of pTV100 (5).
c
C* indicates a T insertion at 1833 bp to inactivate the pasC product (17).