. 1998 Oct;180(20):5463–5465. doi: 10.1128/jb.180.20.5463-5465.1998

TABLE 1.

Bacteria, plasmid vectors, and pas-containing constructs used in this study

Strain, plasmid, or construct	Genotype or description^a	Reference
E. coli strains
JM105	thi rpsL endA sbcB15 hspR4 Δ(lac-proAB) [F′ traD36 proAB lacI^qΔM15]	15
CSH50-I^q	rpsL Δ(lac-pro) [F′ traD36 proAB lacI^qΔM15]	13
Plasmids
pMC1403	ColE1 replicon, Ap^rlacZYA*	2
pACYC184	p15a replicon, Cm^r Tc^r	3
pKK223-3	ColE1 replicon, Ap^rtac	1
pOU82	R1 replicon, Ap^rlacZYA	6
Constructs containing pas genes
pP2H	pMC1403 vector, Ap^r, pas region 1240–1362^b	This work
pTac-pasA-pACYC	pACYC184 vector, Cm^r, pas region 1316–1559	This work
pTac-pasB-pACYC	pACYC184 vector, Cm^r, pas region 1518–1816	This work
pTac-pasC-pACYC	pACYC184 vector, Cm^r, pas region 1789–2028	This work
pTac-pasAB-pACYC	pACYC184 vector, Cm^r, pas region 1316–1816	This work
pTac-pasABC-pACYC	pACYC184 vector, Cm^r, pas region 1316–2028	This work
pKmM0	pTF-FC2 replicon, Km^r, 1–4910^c	12
pKmM1	pTF-FC2 replicon, Km^r, 1–4911 pasA	12
pKmM2	pTF-FC2 replicon, Km^r, 1–4911 pasB	12
pKmM3	pTF-FC2 replicon, Km^r, 1–4911 pasC	12
pKmM1del1^d	pTF-FC2 replicon, Km^r, 1–4911, Δ1217–1321^e	12
pKmM1del2^d	pTF-FC2 replicon, Km^r, 1–4911, Δ1232–1943^e	12
pOU-pasABC	pOU82 R1 replicon, Ap^r, pas region 1158–2027	12
pOU-tac-pasABC	pOU82 R1 replicon, Ap^r, tac, pas region 1158–2027	This work

Ap^r, ampicillin resistance; Km^r, kanamycin resistance; Cm^r, chloramphenicol resistance; Tc^r, tetracycline resistance; lacZYA*, lacZYA genes with deletion of lacZ promoter and ATG; tac, trp-lac hybrid promoter.

The pas region numbers are the nucleotide positions relative to those of the ClaI-PstI fragment of pTV100 (5).

Entire pTF-FC2 replicon from the ClaI site to the PstI site of pTV100 including the pas region (5).

Spontaneous deletions of the toxic plasmid pKmM1 (12).

Regions missing from the spontaneous deletions after PasA antidote inactivation (12).