Fig. 3: Hem25p drives CoQ production independently of its role in haem biosynthesis.
a, Overview of haem biosynthesis and the role of Hem25p. OMM, outer mitochondrial membrane; IMS, inter membrane space; IMM, inner mitochondrial membrane. b, Relative total haem and CoQ abundances in WT and hem25Δ yeast grown in synthetic complete (SC) medium with and without glycine and ALA supplementation (*P = 1.01 × 10−5 WT SC haem versus hem25Δ SC haem, **P = 7.56 × 10−10 WT SC CoQ versus hem25Δ SC CoQ, ***P = 8.47 × 10−8 WT SC CoQ versus hem25Δ+Gly CoQ, ****P = 2.22 × 10−7 WT SC CoQ versus hem25Δ+ALA CoQ; mean ± s.d., n = 3 biologically independent samples, two-sided Student’s t-test). c, Relative protein abundances of WT cells with ALA, hem25Δ cells without ALA, and hem25Δ cells with ALA supplementation compared to WT cells without ALA supplementation versus statistical significance. CoQ biosynthetic proteins and complex Q proteins are highlighted (mean, n = 3 biologically independent samples, two-sided Student’s t-test). d, Relative protein abundances of hem25Δ cells ± ALA supplementation compared to WT cells without ALA supplementation versus statistical significance. Components of the electron-transport chain are highlighted (mean, n = 3 biologically independent samples, two-sided Student’s t-test). e, Relative abundance of de novo synthesized [13C6]-PPHB and [13C6]-CoQ in WT, hem25Δ, coq6Δ and coq6Δhem25Δ cells grown in –pABA SD, 3% (wt/vol) glycerol and 50 μM [phenyl-13C6]-4-HB (mean ± s.d., n = 3 biologically independent samples). ND, not detected. f, Normalized abundance of de novo synthesized [13C12]-PPHB in isolated coq6Δ and coq6Δhem25Δ mitochondria (*P = 5.73 × 10−5 WT versus hem25Δ at 5 min, **P = 0.0001 WT versus hem25Δ at 15 min; mean ± s.d., n = 3 biologically independent samples, two-sided Student’s t-test). Numerical data are available as Source data.