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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Nat Cell Biol. 2023 Oct 9;25(11):1616–1624. doi: 10.1038/s41556-023-01250-5

Fig. 5: Hem25p function is required for bacterial IPP import.

Fig. 5:

a, Multiple sequence alignment of HEM25 orthologues with residues mutated in congenital sideroblastic anaemia highlighted. b, Predicted structure of Hem25p showing the location of the disease-related residues. IMS, intermembrane space. c, Relative CoQ abundances in hem25Δ yeast carrying WT and mutant HEM25FLAGconstructs. Levels are relative to WT yeast carrying the empty expression vector (*P = 8.58 × 10−5, **P = 6.67 × 10−5, ***P = 6.78 × 10−5, ****P = 9.73 × 10−5 HEM25FLAG versus mutants or empty vector, mean ± s.d., n = 3 biologically independent samples, two-sided Student’s t-test). ND, not detected. d, Relative [1-14C]-IPP uptake by E. coli cells expressing WT or mutant MBP-Hem25p. Uptake levels reflect 30 min of incubation time and are relative to that of WT MBP-Hem25p (*P = 0.0063 empty vector versus WT HEM25, mean ± s.d., n = 3 biologically independent samples, two-sided Student’s t-test). NS, not significant. e, Normalized abundance of de novo synthesized [13C16]-OPP in E. coli cells expressing the empty vector (EV), WT MBP-Hem25p or the R181P MBP-Hem25p mutant (*P = 0.0116 WT versus R181P MBP-Hem25p at 5 min, **P = 4.1 × 10−5 WT versus R181P MBP-Hem25p at 15 min, ***P = 0.0001 WT versus R181P MBP-Hem25p at 30 min, mean ± s.d., n = 3 biologically independent samples, two-sided Student’s t-test). Numerical data are available as Source data.