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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Nat Cell Biol. 2023 Oct 9;25(11):1616–1624. doi: 10.1038/s41556-023-01250-5

Extended Data Fig. 2. 4-HB import by pcaK and unadjusted IPP uptake kinetics.

Extended Data Fig. 2

a, Immunoblot for MBP-Hem25p, MBP-SLC25A38, and MBP-Yhm2p in E. coli membranes preparations. b, Steady-state kinetics of [1-14C]-IPP uptake in cells expressing MBP-Hem25p or the empty vector. Initial rates (nmol/min) values represent the mean ± SD of three biologically independent samples. c, Time course of 50 μM [phenyl-14C6]-4-HB uptake by E. coli cells expressing the Pseudomonas putida PcaK or the empty vector (*p = 0.0002, **p = 0.0042, ***p = 0.0006, ****p = 0.0011 empty vector vs pcaK, mean ± SD, n = 3 biologically independent samples, two-sided Student’s t-test). Source numerical data and unprocessed blots are available in source data.