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[Preprint]. 2024 Jun 20:2023.11.28.569014. [Version 3] doi: 10.1101/2023.11.28.569014

Figure 5: Depletion of GSH biosynthesis with BSO impairs regeneration and is rescued by exogenous GSH.

Figure 5:

(A) 7 days post germination (DPG) seedlings (PlaCCI × WIP4) grown on MS (control) or germinated on MS+1mM BSO then stained overnight with blue CMAC. (B) Representative images of the WIP4 signal in a median section of a control and BSO-treated root at 24 and 72 HPA. (C) Representative images of WIP4 signal 24 HPA in control and 0.5 mM BSO treatment. (D) Quantification of WIP4 signal in the regeneration zone of roots 24 HPA in control and 0.5 mM BSO treatment. The y-axis is the scaled corrected total cell Fluorescence. (E) Representative images of regenerating root tips stained with mPS-PI to visualize cell walls and amyloplasts 18 HPC. Cells with amyloplasts are pseudo-colored in yellow. The treatments are control, 0.5 mM BSO, 0.5 mM GSH, or combined 0.5 mM BSO + 0.5 mM GSH. (F) Quantification of the number of cells with amyloplasts in a population of roots from each treatment group shown in E. Pairwise statistical testing was performed using the Wilcoxon test.