Table 1.
Cryo-EM Data Collection and Refinement Statistics
| DH270.6 – DE3 | VRC01 – DE3 | |
|---|---|---|
| Data Collection | ||
| Microscope | Titan Krios | Titan Krios |
| Voltage (kV) | 300 | 300 |
| Electron dose (e−/Å2) | ~60 | ~60 |
| Detector | Gatan K3 | Gatan K3 |
| Pixel Size (Å) | 1.08 | 1.08 |
| Defocus Range (μm) | 0.42-4.71 | 0.47-3.674 |
| Magnification | 105000 | 105000 |
| Reconstruction | ||
| Software | cryoSPARC | cryoSPARC |
| Particles | 236154 | 220881 |
| Symmetry | C3 | C3 |
| Box size (pix) | 300 | 300 |
| Resolution (Å) (FSC0.143)$ | 3.6 | 3.7 |
| Validation | ||
| Protein residues | 2484 | 2457 |
| EMRinger Score | 2.79 | 2.06 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.005 | 0.004 |
| Bond angles (°) | 0.905 | 0.814 |
| Molprobity score | 1.47 | 1.53 |
| Clash score | 2.03 | 2.98 |
| Rotamer outliers (%) | 0.00 | 0.00 |
| Ramachandran | ||
| Favored regions (%) | 92.79 | 93.21 |
| Disallowed regions (%) | 0.49 | 0.12 |
$
Resolutions are reported according to the FSC 0.143 gold-standard criterion