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[Preprint]. 2023 Dec 14:2023.12.13.571184. [Version 1] doi: 10.1101/2023.12.13.571184

Figure 4. Tardbp EC-KO mice exhibit endothelial dysfunction and vascular leak in multiple tissues.

Figure 4.

Phenotypic analysis of TDP-43 EC-KO mice versus littermate controls at 3–4 weeks of age. (A) Mice are moribund by 3–4 weeks post-Tam treatment and gene excision. (B) Vascular leak assessed by Evans blue (EB) dye extraction (tissue EB normalized by plasma EB, presented as a fold change over the same tissue from control mice). (C) Absolute platelet count measured by flow cytometry of blood, normalized to littermate controls. (D, E) Ultrasound with quantitation, demonstrating impaired ejection fraction in EC-KO mice (n=6 EC-KO and 5 littermate controls). (F) Fibrosis was observed in trichrome-stained tissues (blue staining indicates collagen). (C) Statistical analysis was performed using an unpaired two-tailed Student’s t-test. (C&E) Statistical analysis was conducted using the Mann-Whitney test, *P<0.05.