Table 1: AAV_retro fluorophore evaluation.

Fluorophore	Laser	Interfering Fluorophore	Reason for ranking
mOrange2	514 nm	NA	Bright, distinct
mTagBFP2	405 nm	NA	Several distinct bins
mVenus	514 nm	NA	Bright
mTurquoise2	405 nm *Would benefit from 455 nm	mTagBFP2 & T-Sapphire	Neutral
T-Sapphire	405 nm	NA	Neutral
FusionRed	594 nm	mScarlet	Underperformer, slightly dim
mScarlet	561 nm	MOrange2 & mNeptune	Cross talk
mNeptune2.5	639 nm	mScarlet	Dim, cross talk
mCyRFP2	488 nm	NA	Very dim