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[Preprint]. 2023 Dec 14:rs.3.rs-3694849. [Version 1] doi: 10.21203/rs.3.rs-3694849/v1

Figure 5: LMNO fusions recapitulate in vivo Cxcl12-dependent REELIN+ Cajal-Retzius cell migration.

Figure 5:

(A-B) Images of LMNO fusions labeled with (A) neuronal markers HOPX (magenta)/MAP2 (red), (B) progenitor marker PAX6 (red) and human mitochondria (magenta). Top image scale bar 100μm and bottom image scale bar 10μm. (C) Cajal-Retzius cell marker REELIN (red); Col1a1-GFP marking meningeal fibroblasts (green). Top 2 images scale bar 100μm and bottom image scale bar 10μm. (D) Images of in situ detection of Cxcl12 (magenta) in LMNO fusions at 30 and 60 days in culture, Col1a1-GFP marking meningeal fibroblasts (yellow). Yellow-dashed lines distinguish organoid compartment (org.) from meninges (men.). All scale bars 100μm.