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Doxorubicin treatment on 2D and 3D cultures. Two CSA cell line (a) SW1353, (b) HT1080, and six primary cells (c) S166, (d) S494, (e) CS21b, (f) CS23, (g) CS15 and (h) S564 were cultured as both monolayer (2D) and spheroids (3D). Cells were treated by doxorubicin at different dose. Data was fitted by nonlinear curve (four parameters) with least squares. Five independent cultures were treated and measured by PrestoBlue.
