TABLE 2.

Properties of proline mutant PhePs

Residue	No. of family members in which residue is conserveda	% of wild type		Protein leveld
		Steady-state level of accumulationb	Initial ratec
Pro17	1	97	98
Pro47	1	90	92
Pro54	12	50	60	100
Pro82	11	102	97
Pro97	7	98	95
Pro131	11	98	101
Pro134	8	80	78	69
Pro238	13	100	103
Pro243	10	99	101
Pro269	12	72	72	100
Pro279	12	141	136	85
Pro329	11	80	83	84
Pro341	13	5	11	100
Pro363	10	135	129
Pro411	11	80	87	124
Pro442	9	46	42	90

^aIncluding AROP, PROY, YIFK, CYCA, ANSP, YKFD, LYSP, and GABP from E. coli; ROCE, ROCC, and HUTM from Bacillus subtilis; and AROP from Corynebacterium glutamicum. 

^bPercentage of steady-state level of [¹⁴C]phenylalanine uptake compared to the wild type (100% is 10.1 nmol/mg of dry weight). 

^cPercentage of initial rate of [¹⁴C]phenylalanine uptake compared to the wild type (100% is 45.4 nmol/mg of dry weight/min). 

^dValues are percentages of the wild-type protein level derived from immunoprecipitation experiments.