TABLE 3.
Properties of mutant PhePs with substitutions at P54, P341, and P442
Residue | % of wild-type levela (relative protein level)b
|
||||||
---|---|---|---|---|---|---|---|
Gly | Leu | Gln | Ser | Thr | Lys | Arg | |
P54 | 100 | 26 (101) | |||||
P341 | 0 (106) | 0 (83) | 3 (110) | 17 (63) | 0 (73) | 0 (84) | |
P442 | 52 (103) | 43 (121) |
Percentage of the steady-state level of [14C]phenylalanine uptake by the wild type.
Values in parentheses are relative levels of protein detected by immunoprecipitation experiments.