Table 1. Bacterial strains, bacteriophage, plasmids, and oligonucleotide primers.
Strain, plasmid, phage, or primer | Relevant characteristic(s), description, or sequence | Source or reference |
---|---|---|
Strains | ||
A. baumannii ABMYH-1245 | Multidrug-resistant, clinical isolate with KL10; primary host bacteria of phage T1245 | [11] |
A. baumannii ABAPSP-55 | Multidrug-resistant, clinical isolate with KL10 | [11] |
A. baumannii ABAPSP-64 A. baumannii ABMYSP-109 |
Multidrug-resistant, clinical isolate with KL10 Multidrug-resistant, clinical isolate with KL10 | [11] |
A. baumannii ABMYSP-101 | Multidrug-resistant, clinical isolate with KL10 | [11] |
A. baumannii ABMYSP-182 | Multidrug-resistant, clinical isolate with KL10 | [11] |
A. baumannii AB1039 | Multidrug-resistant, clinical isolate with KL2 | [11] |
A. baumannii AB3396 | Multidrug-resistant, clinical isolate with KL2 | [11] |
A. baumannii ABJNH-403 | Multidrug-resistant, clinical isolate with KL3 | [11] |
A. baumannii ABMYSP-185 | Multidrug-resistant, clinical isolate with KL6 | [11] |
A. baumannii ABMYSP-210 | Multidrug-resistant, clinical isolate with KL6 | [11] |
A. baumannii ABMYSP-216 | Multidrug-resistant, clinical isolate with KL6 | [11] |
A. baumannii ABMYSP-419 | Multidrug-resistant, clinical isolate with KL6 | [11] |
A. baumannii ABMYH-1033 | Multidrug-resistant, clinical isolate with KL6 | [11] |
A. baumannii AB15 | Multidrug-resistant, clinical isolate with KL47 | [11] |
A. baumannii ABAPP-61 | Multidrug-resistant, clinical isolate with KL47 | [11] |
A. baumannii AB724 | Multidrug-resistant, clinical isolate with KL49 | [11] |
A. baumannii AB2792 | Multidrug-resistant, clinical isolate with KL49 | [11] |
A. baumannii ABMYSP-21 | Multidrug-resistant, clinical isolate with KL52 | [11] |
A. baumannii ABMYSP-444 | Multidrug-resistant, clinical isolate with KL52 | [11] |
E. coli Top10 | Laboratory strain for TA cloning use | Invitrogen, San Diego, USA |
E. coli BL21 (DE3 | Laboratory strain for protein expression | Invitrogen, San Diego, USA |
Plasmids | ||
pGEM-T-easy | 3,015-bp E. coli vector, Ampr, Plac, lacZ | Promega, San Diego, USA |
pET30b(+) | Expression vector; 5,421-bp E. coli vector, Kmr, PT7, His-Tag | Novagen, Wisconsin, USA |
Phages | ||
A. baumannii phage T1245 | Accession No. ERS3583556 | [28] |
Primers | ||
Forward primer: FP-EcoRIEndo | GAATTCGATGATTCTGACTAAAGACGG | This study |
Reverse primer: RP-XholEndo | CTCGAGTAAGCTCCGTAGAG | This study |