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. 2023 Dec 18;14:1286831. doi: 10.3389/fimmu.2023.1286831

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Copyright © 2023 Cime-Castillo, Vargas, Hernández-Tablas, Quezada-Ruiz, Díaz and Lanz-Mendoza

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Experimental design of the trade-off analysis of the progeny (F1) of priming mothers (F0) with homologous (PrHm), heterologous infection (PrHt) infection with dengue virus serotype 2 (represented in red), 4 (represented in blue) and unprimed mosquitoes (UnPr), which evaluated different biological parameters: 1) Oviposition (number of eggs deposited by individual mothers); 2) Percentage of Hatching (percentage of the number of eggs hatched); 3) Percentage of pupation (percentage of the number of larvae that pupated); 3A) Percentage of pupation by sex (percentage of number of larvae that pupated in both sexes); 3B) Percentage of pupation as a function of time (Percentage of the number of larvae that pupated over time); 4) Percentage of emergence (Percentage of the number of pupae that emerge to adult); 4A) Sex ratio (number of males and females that emerge to adult); 4B) Body size (measurement of the pair of wings of adult mosquitoes) and 4C) Transgenerational immune priming (TGIP) analysis (See Figure 3 ).