Skip to main content
. Author manuscript; available in PMC: 2024 Jan 2.
Published in final edited form as: Biochemistry. 2023 Sep 20;62(24):3533–3547. doi: 10.1021/acs.biochem.3c00371

Figure 1. The scheme of the procedure.

Figure 1.

(A) Schematic showing the injection procedure. MC3 LNPs containing either Cre mRNA or Cas9 mRNA/Ai9 sgRNA injected into the brain were able to deliver mRNA and induce gene recombination or editing in Ai9 mice. (B) Left: Scheme of the injection site for MC3 LNP Cre mRNA and controls (saline or Cre mRNA) in either the striatum or hippocampus of adult Ai9 mice. Saline, Cre mRNA (0.125 μg μL−1 or 0.250 μg μL−1) or MC3 LNP Cre mRNA (0.125 μg μL−1 or 0.250 μg μL−1) was injected into the striatum or hippocampus of Ai9 mice. Right: Scheme of Cre-mediated recombination of tdTomato gene. (C) Left: Schematic showing the injection site for MC3 LNP Cas9 mRNA/Ai9 sgRNA and controls (saline or Cas9 mRNA) in either the striatum or hippocampus of adult Ai9 mice. Saline, Cas9 mRNA (0.125 μg μL−1 or 0.250 μg μL−1) or MC3 LNP Cas9 mRNA/Ai9 sgRNA (0.125 μg μL−1 or 0.250 μg μL−1) was injected into the striatum or hippocampus of Ai9 mice. Right: Scheme of Cas9-mediated editing of tdTomato gene.