Figure 8. The cell type specificity after the injection of MC3 LNP Cas9 mRNA with Ai9 sgRNA in the striatum and hippocampus of Ai9 mice.

(A, C) Immunostaining of tdTomato⁺ (red) and either NeuN⁺ (cyan), GFAP⁺ (green) or Iba1⁺ (green) cells 21 days after stereotaxic injection of MC3 LNP Cas9 mRNA/Ai9 sgRNA (0.250 μg μL⁻¹) in the striatum (A), and the hippocampus (C). The scale bar represents 30 μm. (B, D) Quantification of (B) tdTomato⁺; NeuN⁺, tdTomato⁺; GFAP⁺ or tdTomato⁺; Iba1⁺ cells among total tdTomato⁺ cells (%) in the MC3 LNP Cas9 mRNA/Ai9 sgRNA (0.250 μg μL⁻¹)-injected striatum (B), and hippocampus (D). All data are presented as mean ± SEM. MC3 LNP Cas9 mRNA/Ai9 sgRNA (0.250 μg μL⁻¹; n = 10 images from 2 mice).