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. 2024 Jan 2;134(1):e173564. doi: 10.1172/JCI173564

Figure 6. Capacitation- and progesterone-induced hyperactivation of control and CATSPER2–/– sperm.

Figure 6

(A) Scatter plot (mean ± SD) of the fraction of hyperactivated control sperm from donors (black triangles, n = 17) and CATSPER2–/– patients (color-coded circles, n = 10 experiments with sperm from patients C1, C2, C5, C7, and C8) upon incubation under capacitating conditions. ****P < 0.0001, unpaired t test with Welch´s correction. (B) Paired plots of the fraction of hyperactivated control sperm before (basal) and after treatment (5 minutes) with progesterone (5 μM) determined by CASA (n = 5). (C) Change of the fraction (mean ± SD) of hyperactivated control (black, n = 5) and CATSPER2–/– sperm (blue, n = 3, i.e., 3 experiments with sperm from patient C5) evoked by mixing with progesterone (gray bar), corrected for the fraction of hyperactivated sperm determined after mixing with HTF++ alone (set to 0 at t = 0 seconds), and determined by a custom kinetic CASA technique. Experiments were performed with sperm incubated under capacitating conditions. **P < 0.01, ***P < 0.001, ANOVA with Dunnett’s multiple comparison test versus the respective controls (t = 0 s).