Figure 6. Asxl1^Y588XTg BM cells are sensitive to the KDM6B inhibitor GSK-J4.

(A) Western blot showing the levels of H3K27me3 in BM cells treated with 5 μM GSK-J4 for 24 hours. (B and C) Colony-forming assays using BM cells with or without GSK-J4 treatment (n = 5 mice per genotype). Representative images of colony formation from each condition are shown. The concentration of GSK-J4 is indicated. WT, IC₅₀ = 5.63 μM; Asxl1^Y588XTg, IC₅₀ = 3.86 μM. (D and E) The frequencies of CD45.2⁺ (D) and Gr1⁺Mac1⁺ cells (E) in PB from Asxl1^Y588XTg mice treated with GSK-J4 (n = 9). Data represent the mean ± SEM. *P < 0.05 and ***P < 0.001, by unpaired Student’s t test (C) and paired Student’s t test (D and E).