| Antibodies |
| Rabbit anti-FOS |
Cell Signaling |
No. 2250 |
| Sheep anti-Foxp2 |
R&D Systems |
AF5647 |
| Chicken anti-GFP |
Abcam |
ab13970 |
| Rat anti-mCherry |
Invitrogen |
16D7 |
| Goat anti-nNos |
R&D Systems |
AF2416 |
|
|
|
| Bacterial and virus strains |
| AAV5-EF1a-DIO-ChR2-EYFP |
Penn Vector Core |
N/A |
| AAV2-EF1a-DIO-ChR2-mCherry |
UNC Vector Core |
N/A |
| AAV2-EF1a-DIO-mCherry |
UNC Vector Core |
N/A |
| AAV8-hSyn-DIO-hM4D(Gi)-mCherry |
Addgene |
Addgene number:44362 |
| AAV2-hSyn-DIO-hM3D(Gq)-mCherry |
Addgene |
Addgenenumber: 44361 |
| AAV2-Flex-taCaso3-Tevp |
UNC Vector Core |
N/A |
| AAV5-Flex-taCasp3-Tevp |
UNC Vector Core |
N/A |
| AAV9-syn-dLight1.3b |
Addgene |
Addgene number: 135762 |
| AAV1-syn-FLEX-jGCaMP7s-WPRE |
Addgene |
Addgene number: 104491 |
| AAV9-GFP-U6-scrmb-shRNA |
Vector Biosystems Inc |
No. 7777 |
| AAV9-GFP-U6-m-Ptger3-shRNA |
Vector Biosystems Inc |
No. 7000 |
|
|
|
| Chemicals, peptides, and recombinant proteins |
|
|
| PGE2 |
Sigma |
D2250000/P0409-1MG |
| Furosemide |
Sigma |
F4381 |
| Low-sodium diet |
ENVIGO, TD |
No.90228 |
| CNO |
Sigma |
C0832 |
| Formalin |
Sigma |
HT. 5012 |
| Mannitol |
Sigma |
M9647 |
| PGE2-AMCA |
AAT Bioquest |
Q2303063 |
| Papain |
Sigma |
P3125 |
| Actinomycin D |
Sigma |
A1410 |
| DNase I |
Thermo Scientific |
90083 |
| Ovomucoid inhibitor |
Worthington |
OI-BSA |
| Ghrelin |
Phoenix pharmaceuticals |
031-31 |
|
|
|
| Deposited data |
| scRNA-seq |
Gene Expression Omnibus |
GSE 244245 |
| SeqFish |
Zenodo |
Zenodo 8381359 |
|
|
|
| Experimental models: Organisms/strains |
|
C57BL/6J
|
Jackson Laboratory |
No. 0664 |
|
PdynCre
|
Jackson Laboratory |
No.027958 |
|
Trpm5−/−
|
Jackson Laboratory |
No.013068 |
|
Ptger3Cre
|
Jackson Laboratory |
No.035575 |
|
|
|
| Software and algorithms |
| MATLAB R2022a |
MathWorks |
N/A |
| Prism 9.3.1 |
GraphPad |
N/A |
| LASX |
Leica |
N/A |
| Photoshop CS5/CC |
Adobe |
N/A |
| Illustrator CS5/CC |
Adobe |
N/A |
| Zen Black 2.3 |
| VS-ASW-S6 2.8 |
Olympus |
N/A |
| Python 3.9.12 |
Python Software Foundation |
N/A |
| R 4.1.2 and 4.1.1 |
R Core Team |
N/A |
