TABLE 2.
P oxidation phenotypes of plasmid-complemented P. stutzeri mutantsa
| Plasmid | Phenotypeb of strain:
|
|||||
|---|---|---|---|---|---|---|
| WM581 [del3(BsiWI)]
|
WM691 [del2(SstI-KpnI)]
|
WM688 [del1(SstI-BsiWI)]
|
||||
| Hpt | Pt | Hpt | Pt | Hpt | Pt | |
| None | − | − | − | + | − | − |
| pWM239 | + | + | + | + | + | +/− |
| pWM276 | + | + | + | + | + | +/− |
| pWM275 | + | + | + | + | + | +/− |
| pWM278 | + | + | + | + | + | + |
| pWM280 | + | + | − | + | − | + |
| pWM287 | + | + | − | + | − | + |
| pWM288 | + | + | − | + | − | + |
| pWM269 | − | − | + | + | − | − |
| pWM279 | − | − | + | + | − | − |
| pWM286 | − | − | + | + | − | − |
| pWM281 | − | − | − | + | − | − |
Strains tested were exconjugates of the indicated recipient P. stutzeri strains with E. coli S17-1 donors carrying the indicated broad-host-range plasmid. See Fig. 2 for the physical structure of each plasmid and Fig. 3 for the extents of the indicated deletions.
Oxidation of reduced P compounds to phosphate was scored by testing each strain for growth on 0.4% glucose–MOPS medium with 0.5 mM hypophosphite (Hpt) or phosphite (Pt) as the sole P source. Because all organisms require phosphate, growth on these media is indicative of the ability to oxidize the P compound included as a P source. +, growth within 24 h at 37°C; +/−, growth within 72 h; −, no growth within 72 h. All strains tested grew in the identical medium supplemented with 0.5 mM phosphate.