Figure 3.

EIF4A3 suppresses the expression of hsa_circ_0001445 in LSCC.

(a) The diagram shows the binding sites of EIF4A3 on the flanking region of hsa_circ_0001445 predicted by Circular RNA Interactome. (b) The RIP assay was performed to verify the binding sites of EIF4A3 on the flanking region of hsa_circ_0001445. (c) Using GEPIA to analyze the expression of EIF4A3 in head and neck squamous cell carcinoma and its effect on patients’ survival. (d) Western blot analysis was used to verify the successful overexpression of EIF4A3 in LSCC cells, and qRT-PCR was used to detect the expression of hsa_circ_0001445 and SMARCA5 after overexpression of EIF4A3. Data represent means ± SD of three independent experiments. *P< 0.05, **P< 0.01, ***P< 0.001.