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. 2023 Nov 23;22(18):2038–2056. doi: 10.1080/15384101.2023.2274670

Figure 4.

Figure 4.

Hsa_circ_0001445 acts as a sponge for hsa-miR-432-5p in LSCC.

(a) RIP assay was carried out with AGO2 antibody in TU177 and AMC-HN-8 cells, and then the enrichment of hsa_circ_0001445 was detected by qRT-PCR. (b) Combined analysis of two online databases (Circular RNA Interactome and Starbase 3.0) to screen for hsa_circ_0001445-binding miRNAs, p = 1.782164e-06. (c) Relative level of hsa_circ_0001445 in TU177 and AMC-HN-8 lysates after RNA pull down using hsa_circ_0001445 probe or NC probe. And relative level of two miRNAs in TU177 and AMC-HN-8 lysates pulled down by hsa_circ_0001445 probe or NC probe. (d) HEK293T cells were co-transfected with hsa-miR-432-5p mimics and wild-type (hsa_circ_0001445-WT) or mutant (hsa_circ_0001445-MUT) hsa_circ_0001445 luciferase reporter vector, and luciferase activity was detected. Data represent means ± SD of three independent experiments. *P< 0.05, **P< 0.01, ***P< 0.001. NC, negative control.