Figure 7.

Silencing RGMA attenuates the inhibitory effect of hsa_circ_0001445 on LSCC cells.

(a) QRT-PCR and western blot analysis were used to verify the successful knockdown of RGMA in LSCC cells. (b) & (c) MTS and colony formation assays indicated that cell proliferation ability of TU177 and AMC-HN-8 cells transfected with overexpression plasmid of hsa_circ_0001445 was reversed when co-transfected with RGMA siRNA. (d & e) Transwell assays demonstrated that cell migration and invasion abilities of TU177 and AMC-HN-8 cells transfected with overexpression plasmid of hsa_circ_0001445 were counteracted when co-transfected with RGMA siRNA. Data represent means ± SD of three independent experiments. *P< 0.05, **P< 0.01, ***P< 0.001.