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. 2023 Dec 21;16(1):2295384. doi: 10.1080/19490976.2023.2295384

Figure 7.

Figure 7.

F. nucleatum increases HT-29 proliferation and activates anti-apoptotic but not autophagic genes expression via the TIFA pathway. (a) WT (left) or TIFA−/− (right) HT-29 cells were left untreated (control), stimulated with ADP-H (10−6M), control media or F. nucleatum supernatant prior treatment with 5-fluoroucil (5-Fluo, 100 μM, gray bars) or without treatment (black bars). Cell viability HT-29 cell viability was monitored with MTS assay and normalized to the MTS response of untreated cells. B-E. Real-time qPCR (RT-qPCR) showing BIRC3 (b), TNAIP3 (c), ICAM-1 (d), ATG7 (e), ULK1 (f) and CCND1 (g) relative expression to GAPDH in WT (left) or TIFA−/− (right) HT-29 cells, untreated (control), stimulated with ADP-H (10−6M), control media or F. nucleatum supernatant for 6 h. Results were normalized on GAPDH and expressed as 2−ΔΔCt toward unstimulated cells. Data represent ≥ 3 independent experiments. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test. ****P < .0001; ***P < .001; **P < .01; *P < .05; P <.05 was considered as not significant (ns).