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. 2023 Aug 23;20(1):45–57. doi: 10.1080/15548627.2023.2247310

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — The positive effect of MTOR inhibition on autophagy deficient HSCs is cell intrinsic. (A) Experimental setup of rapamycin treatment in Mx1^Cre atg5^fl/fl:WT (1:1) mixed BM chimeras. (B) Frequencies of PTPRC^b/CD45.2 cells in the blood of chimeric mice 10 weeks after reconstitution, prior to poly(I:C) treatment. (C) Frequencies of PTPRC^b/CD45.2 LSKs. (D) Frequencies of PTPRC^b/CD45.2 HSCs. (E) MFI of p-RPS6 (PTPRC^b/CD45.2: PTPRC^a/CD45.1 ratio) in LSKs. (F) MFI of p-RPS6 (PTPRC^b/CD45.2: PTPRC^a/CD45.1 ratio) in HSCs. (G) MFI of OPP (PTPRC^b/CD45.2: PTPRC^a/CD45.1 ratio) in LSKs. (H) MFI of OPP (PTPRC^b/CD45.2: PTPRC^a/CD45.1 ratio) in HSCs. (I) MFI of MKI67 in PTPRC^b/CD45.2 LSKs. (J) MFI of MKI67 in PTPRC^b/CD45.2 HSCs. (K) MFI of cystine-FITC (PTPRC^b/CD45.2: PTPRC^a/CD45.1 ratio) in LSKs. (L) MFI of cystine-FITC (PTPRC^b/CD45.2: PTPRC^a/CD45.1 ratio) in HSCs. (M) MFI of Kyn in PTPRC^b/CD45.2 LSKs. (N) MFI of Kyn in PTPRC^b/CD45.2 HSCs. Pooled data from 2 experiments (n = 6 animals/group). Data are represented as mean ± SEM with two-way ANOVA with post hoc Tukey’s test.