TABLE 2.
Expression of β-galactosidase from an aotJ::lacZ fusion in PAO1 and PAO501
| Host strain (genotype)a | Growth conditionb | Sp act (nmol/mg/ml)c |
|---|---|---|
| PAO501 (argR::Gm) | S+N | 940 (47) |
| G | 1,030 (31) | |
| G+A | 1,038 (50) | |
| PAO1 (wild type) | S+N+A | 6,021 (310) |
| S+N | 1,654 (31) | |
| S+A | 9,586 (470) | |
| G | 3,662 (37) | |
| N+A | 42,368 (420) | |
| G+A | 23,928 (240) | |
| A | 44,436 (430) |
a
The aot::lacZ translational fusion, pST500, was constructed and transformed into PAO1 and PAO501 as described in Materials and Methods.
b
Cells were grown in minimal medium P supplemented with 20 mM succinate (S), ammonium (N), glutamate (G), and arginine (A) as indicated.
c
Specific activities represent the average of cultures under each growth condition, with standard errors being shown in parentheses. The values shown are normalized relative to plasmid DNA.