Figure 4.

PLLA/HA@Irisin membranes promoted the in vitro osteogenic differentiation of BMMSCs. (A, B) The expression level of COL I was evaluated by immunofluorescence staining, n = 3. Scale bar = 50 μm. (C) After 7 days of osteogenic induction, ALP staining was performed on BMMSCs scale bar = 100 μm. (D) Quantitative results of ALP activity of BMMSCs, n = 3. (E) Representative images of bone mineral deposition in BMMSCs stained by ARS. Scale bar = 100 μm. (F) Quantification of the stained bone mineral deposition in BMMSCs cultured on different groups of membranes, n = 3. (G) The gene expression of osteogenic makers, including Col1a1, Sp7, and Runx2, was determined by RT-PCR, n = 4. (H, I) The protein levels of COL I, SP7 and RUNX2 were examined by western blot, n = 3. Statistically significant differences were indicated by *P < 0.05 or **P < 0.01.