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. 2023 Oct 31;11:rbad096. doi: 10.1093/rb/rbad096

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — PLLA/HA@Irisin membranes enhanced the antioxidant functions of BMMSCs. (A) Mitochondrial and intracellular ROS were detected through MitoSOX and DCFH-DA staining, respectively. Scale bar = 50 μm. (B) Quantification of mitochondrial ROS in BMMSCs, n = 3. (C) Quantification of intracellular ROS in BMMSCs, n = 3. (D) The gene expression of Sirt3, Sod2 and Gpx1 was determined by RT-PCR. (E, F) Western blot was used to determine the protein levels of SIRT3, SOD2 and GPX1. Statistically significant differences were indicated by *P < 0.05 or **P < 0.01.