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. 2023 Oct 31;11:rbad096. doi: 10.1093/rb/rbad096

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Histological and immunohistochemical analysis of the newly formed bone tissue in the calvarial defects. (A) H&E images of the defect area at 4 and 8 weeks post-surgery. (B) MTS images of the defect area at 4 and 8 weeks post-surgery. (C) IHC images of COL I in the defect area. (D) IHC images of CD31-positive cells that represented the newly formed blood vessels. Scale bar = 100 μm. (E) Quantification of the COL I-positive area, n = 3. (F) Quantification of the CD31-positive cells, n = 3. Statistically significant differences are indicated by *P < 0.05 or **P < 0.01.