Table 4.

IC₅₀ value in the L929 cell line treated with different concentrations of W. somnifera leaf methanolic extract.

Effect of leaf methanolic extract of W. somnifera in L929 cell line
Concentration (μg/mL)	Absorbance	% cell survival	% inhibition
Control	0.4387 ± 0.0002	99.99 ± 0.005	0
20	0.3175 ± 0.0003	72.37 ± 0.030	27.63 ± 0.015
50	0.243 ± 0.0056	55.39 ± 0.025	44.61 ± 0.020
100	0.1859 ± 0.0005	42.38 ± 0.30	57.62 ± 0.045
200	0.143 ± 0.0030	32.6 ± 0.020	67.40 ± 0.028
500	0.1152 ± 0.0001	26.25 ± 0.035	73.75 ± 0.035
1000	0.1164 ± 0.0003	26.53 ± 0.02	73.47 ± 0.032
1500	0.1166 ± 0.0001	26.59 ± 0.020	73.41 ± 0.030
2000	0.1164 ± 0.0003	26.53 ± 0.045	73.47 ± 0.045
Log IC50 value	1.896 ± 0.0050
IC50 value	78.77 ± 0.795 μg/mL

IC₅₀ value in bold indicates not significant at p ≤ 0.05.