Table 5.

IC₅₀ value in the L929 cell line treated with different concentrations of W. somnifera stem methanolic extract.

Effect of stem methanolic extract of W. somnifera in L929 cell line
Concentration (μg/mL)	Absorbance	% cell survival	% inhibition
Control	0.4387 ± 0.0005	99.99 ± 0.005	0
20	0.3157 ± 0.0003	71.97 ± 0.052	28.03 ± 0.580
50	0.2583 ± 0.0004	58.87 ± 0.040	41.13 ± 0.030
100	0.1999 ± 0.0014	45.57 ± 0.037	54.43 ± 0.036
200	0.1483 ± 0.0007	33.81 ± 0.045	66.19 ± 0.055
500	0.1189 ± 0.0001	27.1 ± 0.068	72.9 ± 0.340
1000	0.1134 ± 0.0003	25.85 ± 0.037	74.15 ± 0.112
1500	0.1131 ± 0.0005	25.77 ± 0.055	74.23 ± 0.060
2000	0.1138 ± 0.0004	25.94 ± 0.026	74.06 ± 0.119
Log IC50 value	1.957 ± 0.004
IC50 value	90.55 ± 0.800 μg/mL

IC₅₀ value in bold indicates not significant at p ≤ 0.05