Table 6.

IC₅₀ value in the L929 cell line treated with different concentrations of W. somnifera root methanolic extract.

Effect of root methanolic extract of W. somnifera in L929 cell line
Concentration (μg/mL)	Absorbance	% cell survival	% inhibition
Control	0.4387 ± 0.0004	99.99 ± 0.005	0
20	0.342 ± 0.0032	77.96 ± 0.030	22.04 ± 0.141
50	0.3199 ± 0.0011	72.91 ± 0.040	27.09 ± 0.045
100	0.2973 ± 0.0014	67.77 ± 0.060	32.23 ± 0.035
200	0.2759 ± 0.0022	62.88 ± 0.045	37.12 ± 0.020
500	0.2157 ± 0.0004	49.16 ± 0.030	50.84 ± 0.036
1000	0.1540 ± 0.0003	35.1 ± 0.041	64.9 ± 0.042
1500	0.1251 ± 0.0003	28.52 ± 0.040	71.48 ± 0.057
2000	0.1179 ± 0.0005	26.87 ± 0.058	73.13 ± 0.030
Log IC50 value	2.558 ± 0.003
IC50 value	361.70 ± 0.795 μg/mL

IC₅₀ value in bold indicates not significant at p ≤ 0.05.