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. 2023 Dec 4;25(1):142–154. doi: 10.1038/s41590-023-01690-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Representative histogram plot of surface CD55 on MZ B cells and follicular B cells. b,c, Representative flow cytometry profiles (b) and frequencies of MZ B cells in total CD19⁺ B cells (c; left) and in total splenocytes (c; right) in Cd55^−/− (n = 18) and control (n = 18) mice. d, Representative distribution patterns of IgM^hi MZ B cells (red) relative to IgD^hi follicular B cells (blue) in spleens of mice of the indicated genotypes; scale bar, 200 μm. Sections are representative of multiple cross-sections from at least three mice of each type. e, Mixed (50:50) BM chimeras were made with CD45.1 WT and CD45.2 Cd55^+/+ or Cd55^−/− BM cells. Frequencies of MZ B cells of the indicated genotype CD19⁺ B cells in WT:Cd55^−/− (n = 6) and control (n = 6) mixed BM chimeras. f, Mixed (50:50) BM chimeras were made with CD45.1 WT and CD45.2 WT (n = 6), Adgre5^−/−Cd55^−/− (dKO; n = 6) or Cd55^−/− and dKO (n = 5) BM cells. Frequencies of MZ B cells among the indicated genotype CD19⁺ B cells in mixed BM chimeras. g, RBC transfusions were performed from Cd55^−/− or Cd55^+/+ mice to the indicated recipient mice once per week, and analysis was performed after 4 weeks. Frequencies of MZ B cells in total B cells (left) and in total splenocytes (right) in mice with purified RBCs transfused as indicated (n = 10 or 11 recipients in each group) are shown. h,i, Mixed chimeras were made with 85% Mpl^−/− and 15% Cd55^+/+ or Cd55^−/− BM cells. A representative histogram shows surface CD55 on platelets (left) and RBCs (right) in chimeras (h). Frequencies of MZ B cells in total splenocytes in Mpl^−/−:Cd55^−/− (n = 7) and control (n = 8) chimeras are shown (i). Data are pooled from five (c), two (e, f and i) or three (g) independent experiments. Each symbol represents one mouse, and lines denote means. Statistical significance was tested by two-tailed t-test (c and i) or two-way ANOVA followed by Sidak’s multiple-comparisons test (e and f) or one-way ANOVA followed by Tukey’s multiple-comparisons test (g).

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