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. 2023 Dec 4;25(1):142–154. doi: 10.1038/s41590-023-01690-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Frequencies of MZ B cells in splenic B220⁺ B cells in WT mice 3 hours treatment with anti-CD97 antibody (n = 8) or saline (n = 8). b, Frequencies of FO B cells in spleen of WT mice 4 days after treatment with anti-CD97 antibody (n = 12) or saline (n = 13). c,d, Representative histogram plots (c) and MFI (d) of cell surface integrins on MZ B cells in Adgre5^−/− (n = 6) and control (n = 6) mice. e,f, Representative histogram plots (e) and MFI (f) of cell surface integrins in WT mice after 4 days treatment with anti-CD97 antibody (n = 6) or saline (n = 6). g-j, Adgre5^+/+ GFP⁺ (g, h) and Adgre5^–/– GFP⁺ (i, j) splenocytes were transferred into WT recipients and the number of donor MZ B cells in blood and spleen determined over time. (g,h) Representative flow cytometry profiles of MZ B cells among GFP⁺ B cells (g) and cell counts (Mean ± SEM) of GFP⁺ MZ B cells staying in blood (h left panel) and homing into the spleen (h right panel) were analyzed at indicated time points (n = 7 on 0 h, n = 8 on 0.17 h, n = 8 on 1 h, n = 8 on 3 h, n = 8 on 6 h, n = 7 on 18 h). (i,j) Representative flow cytometry profiles of MZ B cells among GFP⁺ B cells (i) and cell counts (Mean ± SEM) of GFP⁺ MZ B cells staying in blood (j left panel) and homing into the spleen (j right panel) were analyzed at indicated time points (n = 6 on 0 h, n = 8 on 0.17 h, n = 8 on 1 h, n = 8 on 3 h, n = 7 on 6 h, n = 8 on 18 h). The blood volume in all recipients was estimated as 2 ml. k, Representative histogram plots of surface CD97 on Thy1.1⁺ CD45.2⁺ MZ B cells in chimeras reconstituted as indicated. See corresponding Fig. 2k. Data are pooled from two (a, d, f, h, j) or three (b) independent experiments. Each symbol represents one mouse and lines denote means. Statistical significance was tested by two-tailed t-test (a, b) or two-way ANOVA followed by Sidak’s multiple comparisons test (d, f).

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