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. 2023 Dec 14;34:17–36. doi: 10.1016/j.bioactmat.2023.11.020

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 8 — NAMPT activation protects against AAAin vitroandin vivo

(A) The scheme to study the function of NAMPT activation by its activator P7C3 in AAA pathogenesis. The concentration of P7C3 injected into mouse was 5 μM.

(B) NAD⁺ expression level analysis in mouse abdominal aortas. CTRL, control. CTRL, N = 5; AngII, N = 4; P7C3, N = 5; p**<0.01.

(C) The snapshot images of mouse abdominal aortas. Scale bar, 5 mm. Red arrows showed the phenotype of abdominal aortic aneurysm.

(D) The SA-beta-gal staining of mouse abdominal aortas. Scale bar, 5 mm.

(E–F) Ultrasound evaluation of mouse abdominal aortas. Red arrows showed the maximal aortic diameter. CTRL, control (Sham), N = 5; AngII, N = 4; P7C3, N = 5; p*<0.05; p**<0.01. (G) HE staining of mouse abdominal aorta sections. HE, hematoxylin and eosin. Scale bar, 100 μm.

(H) The SA-beta-gal staining of human AAA VSMCs. The concentration of P7C3 treated on cells was 5 μM. N = 3; p**<0.01. Scale bar, 100 μm.

(I) NAD⁺ expression level analysis in human AAA VSMCs. The concentration of P7C3 treated on cells was 5 μM. N = 3; p**<0.01. (J–K) Western-blotting showing the expression levels of markers associated with senescence (p21, p16) and VSMCs contractile phenotype. The concentration of P7C3 treated on cells was 5 μM. N = 3; p*<0.05, p**<0.01, p***<0.001.

(L) Western-blotting showing the expression of SIRT1 on human control and AAA VSMCs. N = 3; p***<0.001.

(M–N) The SA-beta-gal staining of human control VSMCs. EX-527, a selective SIRT1 Inhibitor. The concentration of P7C3 treated on cells was 5 μM. The concentration of EX527 treated on cells was 10 μM. N = 3; p**<0.01, p***<0.001. Scale bar, 100 μm.

(O) NAD⁺ expression level analysis in human control VSMCs. EX-527, a selective SIRT1 Inhibitor. The concentration of P7C3 treated on cells was 5 μM. The concentration of EX527 treated on cells was 10 μM. N = 3; p*<0.05, p**<0.01.

(P) Western-blotting showing the expression levels of markers associated with senescence (p21, p16) and SIRT1 in human control VSMCs. N = 3; p*<0.05, p**<0.01, p***<0.001.