Table 1.

Effect of addition of different chitosan (CS) concentrations and different CS: TAN-NLCs ratios on the colloidal properties of different prepared CS-TAN-NLCs

Formulation code	CS concentration (% w/v)	CS: TAN-NLCs (v/v)	Particle size (nm)	PDI	ζ-potential (mv)
F1	0	-	152.2 ± 2.9	0.2 ± 0.01	−14.4 ± 0.2
F2	0.1	1:10	178.0 ± 2.8	0.8 ± 0.01	−1.7 ± 0.3
F3	0.1	2:10	190.1 ± 2.4	0.78 ± 0.04	2.2 ± 0.9
F4	0.1	3:10	245.0 ± 6.2	0.47 ± 0.03	7.9 ± 0.3
F5	0.2	1:10	183.4 ± 0.9	0.46 ± 0.04	8.3 ± 0.4
F6	0.2	2:10	194.4 ± 3.0	0.41 ± 0.02	9.8 ± 0.5
F7	0.2	3:10	236.7 ± 6.9	0.34 ± 0.01	17.3 ± 0.6
F8	0.5	1:10	196.1 ± 1.0	0.32 ± 0.01	20.8 ± 0.8
F9	0.5	2:10	245.3 ± 3.5	0.34 ± 0.02	26.0 ± 0.7
F10	0.5	3:10	291.3 ± 1.5	0.33 ± 0.01	29.4 ± 0.5

Data are expressed as mean ± SD (n = 3); average EE% of all prepared formulations was ~98 ± 1.2%