Table 3.
Effect of tested TAN-NLC formulations and TAN suspension on different behavioral and locomotor tests after intranasal administration (0.3 mg/kg) to rotenone-induced PD rat model
| Parameter | Negative control | Positive control | TAN suspension | TAN-NLCs | CS-TAN-NLCs |
|---|---|---|---|---|---|
| Rotarod test | |||||
| Mean latency to fall (s) | 141.7a ± 20.1 | 17.8d ± 2.3 | 70.1c ± 2.8 | 92.1b ± 5.8 | 129.2a ± 21.6 |
| Open field | |||||
| Latency to move (s) | 4.5d ± 0.5 | 10.1a ± 0.5 | 7.4b ± 0.3 | 6c ± 0.3 | 4d ± 0.4 |
| Number of crossed squares | 70.2a ± 3.9 | 24.2d ± 5 | 51.5c ± 5.4 | 60.1b ± 2.5 | 67.3a ± 3 |
| Latency to rear (s) | 10.5c ± 1.9 | 16.4a ± 1 | 14.3b ± 0.9 | 11.3c ± 0.4 | 9.8c ± 1.5 |
| Number of rears | 18.5a ± 3 | 7c ± 1.4 | 12.5b ± 1 | 16a ± 1.4 | 18.3a ± 2.7 |
| Forced swimming test | |||||
| Activity time (s) | 162.5a ± 4.2 | 98.8e ± 3.3 | 140d ± 2.1 | 132.5c ± 2.4 | 157b ± 3.8 |
| Immobility time (s) | 17.5e ± 4.23 | 81.2a ± 3.3 | 47.5b ± 2.4 | 40c ± 2.1 | 23d ± 3.9 |
The study was conducted on male Wistar rats (n = 7). Values were expressed as means ± SD. Data was analyzed using one-way ANOVA followed by post hoc test (Tukey) for group comparisons. Means of similar symbols are statistically insignificant: a > b > c > d > e (p ≤ 0.001)