FIGURE 3.

Conditional loss of club cell Creb1 modifies mucin secretion characteristics. (A) Abundance of mRNA in the murine lung for (A) Rab3D, * = main effect of genotype across treatment, p = 0.0280; (B) P2ry2, * = compared to vehicle control, p = 0.0233; Significant genotype x treatment interaction, p = 0.0142; (C) M3R, * = main effect of genotype across treatment, p = 0.0179; and (D) Muc4, * = main effect of IL-1B treatment across genotype, p = 0. 0048. (D) Density of goblet cells staining for Alcian-Blue/PAS under basal and methacholine stimulated conditions. Density is for cells with acidic mucins, as detected by dark blue staining. Main effect of methacholine, p < 0.0001. (E) Density of goblet cells staining for Alcian-Blue/PAS under basal and methacholine stimulated conditions. Density is for cells with neutral mucins, as detected by purple/magenta staining. Main effect of methacholine, p = 0.0036. Significant treatment x genotype interaction, p = 0.0069. * = compared to Creb1^fl/flScgb1a1^wt vehicle control under methacholine stimulated conditions, p = 0.0028; ** = to Creb1^fl/flScgb1a1^wt IL-1B under methacholine stimulated conditions, p = 0.0067. Post methacholine bronchoalveolar lavage concentrations of (F) Muc5ac and (G) Muc5b. (H) Mean immunohistochemistry signal intensity of Muc5b in the airway post methacholine stimulation. For all panels, individual points are data collected from a single mouse. For Panel F, one mouse from the Creb1^fl/flScgb1a1^cre + VEH group had Muc5ac concentrations below detection and was therefore not included in analysis. Abbreviations: WT, wild type; Scgb1a1^cre, club cell promoter driving CRE recombinase; IL-1B, Interleukin 1β; VEH, vehicle; MCh, methacholine.