TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or property | Source or reference |
|---|---|---|
| RP437 | Wild type for motility and chemotaxis | J. S. Parkinson |
| DFB190 | fliM null strain | 27 |
| RP3098 | De(flhC-flhA) (expresses no chromosomal flagellar genes) | J. S. Parkinson |
| RP5232 | De(cheY) | J. S. Parkinson |
| BL21(DE3) | Host for T7 expression vectors | 26 |
| pTM30 | Protein expression vector, parent of pHT32, pDFB72 and pMn linker-insertion plasmids | 18 |
| pDFB72 | FliM expression vector; Apr | 27 |
| pHT32 | FliM expression vector; Apr | This work |
| pHT41 | fliM in pAlter-1, vector for site-directed mutagenesis | 27 |
| pHT96 | GST-FliN expression vector; Kmr | 29 |
| pHT97 | GST-FliG expression vector; Kmr | 29 |
| pHT100 | GST-only expression vector; Kmr | 29 |
| pHT111 | CheY expression vector; Apr | This work |
| pHT121 | GST-CheY expression vector; Kmr | This work |
| pM16 | Insertion of Pro linker and linker L43 at fliM codon 16 in pDFB72; Apr | This work |
| pM38 | Linker (L42) insertion at fliM codon 38 (BsiWI site) in pHT32; Apr | This work |
| pM60 | Linker (L43) insertion at fliM codon 60 (Eco47III site) in pHT32; Apr | This work |
| pM81 | Pro linker insertion at fliM codon 81 in pHT32; Apr | This work |
| pM111 | Insertion of Pro linker and linker L43 at fliM codon 111 in pHT32; Apr | This work |
| pM132 | Pro linker insertion at fliM codon 132 in pHT32; Apr | This work |
| pM144 | Linker (L40) insertion at fliM codon 144 (NruI site) in pHT32; Apr | This work |
| pM163 | Pro linker insertion at fliM codon 163 in pHT32; Apr | This work |
| pM212 | Insertion of Pro linker and linker L43 at fliM codon 212 in pHT32; Apr | This work |
| pM227 | Pro linker insertion at fliM codon 227 in pHT32; Apr | This work |
| pM241 | Linker (L43) insertion at fliM codon 241 in pHT32; codon 241 also changed from Asn to Asp; Apr | This work |
| pM258 | Linker (L40) insertion at fliM codon 258 (PvuII site) in pHT32; Apr | This work |
| pM267 | Linker (L40) insertion at fliM codon 267 (EcoRV site) in pDFB32; Apr | This work |
| pM282 | Linker (L41) insertion at fliM codon 282 (AatII site) in pHT32; Apr | This work |
| pM310 | Insertion of Pro linker and linker L43 at fliM codon 310 in pHT32; Apr | This work |
| pDFB81 | FliMΔ1–38 expression vector; Apr | This work |
| pHT17 | FliMΔ1–60 expression vector; Apr | This work |
| pHT67 | FliMΔ61–144 expression vector; Apr | This work |
| pHT134 | FliMΔ145–241 expression vector; Apr | This work |
| pHT126 | FliMΔ241–334 expression vector; Apr | This work |
| pHT127 | FliMΔ1–241 expression vector; Apr | This work |
| pMAM1 | FliMΔ61–334 expression vector; Apr | This work |
| pMAM2 | FliMΔ81–334 expression vector; Apr | This work |
| pMAM3 | FliMΔ112–334 expression vector; Apr | This work |
| pMAM4 | FliMΔ145–334 expression vector; Apr | This work |
| pMAM5 | FliMΔ61–240 expression vector; Apr | This work |
| pPB3 | FliMΔ1–247 expression vector; Apr | This work |