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Secretion of ChiA by V. cholerae depends on epsE. (A) Late-log-phase culture; (B) stationary-phase culture. M14 is an epsE mutant derived from 569B. pTDCepsE is a clone of the wild-type epsE gene from 569B. pTDCC2 and pBluescriptKS− were expressed in E. coli DH5αF′tet. Proteins in the immunoblot were detected with a rabbit anti-ChiA hyperimmune serum. S, culture supernatant; P, periplasmic extract. Molecular mass standards are in kilodaltons.
