TABLE 3.
DNA content per nucleoid of uninucleoid and binucleoid cells as determined by RNase plus berberine treatment for four of six size groups of E. fishelsonia
Group | DNA content per nucleoid of uninucleoid cells | Size of nucleoid measuredb | DNA content per nucleoid of binucleoid cells | Binucleoid total DNA | L/S ratioc | Bi.total/uni. ratioc |
---|---|---|---|---|---|---|
I | 20.3 (0.8) | Large | 10.4 (0.3) | 18.4 (0.4) | 1.3 (0.05) | 0.96 |
Small | 8.0 (0.2) | |||||
III | 70.4 (2.1) | Large | 43.8 (0.7) | 81.6 (1.4) | 1.2 (0.02) | 1.19 |
Small | 37.9 (0.7) | |||||
V | 272.6 (3.9) | Large | 145.6 (1.0) | 282.4 (1.3) | 1.1 (0.01) | 1.04 |
Small | 136.8 (0.8) | |||||
VI | 551.8 (11.0) | Large | 298.2 (2.5) | 564.3 (4.2) | 1.1 (0.01) | 1.04 |
Small | 266.2 (2.8) |
a Forty uninucleoid and 40 binucleoid cells of each size group were examined. Binucleoid total DNA is the mean sum of DNA content of large and small binucleoid cells. The L/S ratio is the ratio of DNA content of large binucleoid cells/DNA content of small binucleoid cells. The Bi.total/uni. ratio is the ratio of binucleoid total DNA/uninucleoid cell DNA content. Values are means (standard errors). DNA content is given in arbitrary units.
b Differences between means of small and large nucleoids were significant at <0.001 level for all size groups (paired-sample t test).
c Ratios were not significantly different from 1.0; chi-square test, χ20.05[39] = 54.6 (for L/S ratios) or χ20.05[3] = 7.8 (for Bi.total/uni.ratios).