TABLE 4.
Values of R/G fluorescence ratios in the nucleoids, cytoplasm, and peripheral areas of E. fishelsonia
| Nucleoid type | Mean R/G ratio | SD | SE | 95% confidence interval | Minimum R/G ratio | Maximum R/G ratio | DNA content (arbitrary units) |
|---|---|---|---|---|---|---|---|
| Apical with condensed DNA | |||||||
| Nucleoid | 128.3 (124.8) | 7.8 (7.2) | 0.5 (0.5) | 1.0 (1.0) | 107 (115) | 210 (136) | 247 (197) |
| Cytoplasm | 184.4 (0) | 6.9 | 0.2 | 0.5 | 174 (0) | 210 (0) | 812 |
| Periphery | 151.8 (151.4) | 2.0 (1.9) | 0.1 (0.2) | 0.2 (0.3) | 150 (150) | 154 (154) | 460 (135) |
| Elongate with decondensed DNA | |||||||
| Nucleoid | 145.2 (136.7) | 3.4 (2.1) | 0.2 (0.2) | 0.4 (0.3) | 140 (133) | 148 (139) | 232 (138) |
| Cytoplasm | 210.6 (0) | 32.4 | 1.2 | 2.3 | 182 (0) | 267 (0) | 732 |
| Periphery | 151.6 (150.9) | 2.0 (1.7) | 0.1 (0.2) | 0.2 (0.3) | 150 (150) | 154 (154) | 393 (131) |
| Capped with highly condensed DNA | |||||||
| Nucleoid | 111.4 (112.1) | 13.4 (3.9) | 1.0 (0.4) | 1.9 (0.8) | 92 (96) | 132 (115) | 187 (86) |
| Cytoplasm | 204.2 (0) | 24.1 | 1.0 | 2.0 | 182 (0) | 250 (0) | 547 |
| Periphery | 151.5 (151.1) | 2.6 (1.2) | 0.1 (0.2) | 0.2 (0.3) | 148 (150) | 154 (154) | 434 (135) |
a Values in parentheses are measurements on different cells taken from the same samples and treated with RNase before scanning with a microfluorometer. See Fig. 3 to 6 for distributions within cells at various stages in the life cycle of condensed and decondensed DNA, general and RNA-enriched cytoplasm, and peripheral materials. Note the stability of measurements for peripheral materials at all stages, in contrast to changes in degree of condensation of DNA (low ratios indicate high condensation) in the nucleoids and enrichment of cytoplasm by RNA (higher values result from enrichment).