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. 2024 Jan 2;15:79. doi: 10.1038/s41467-023-44412-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a PI-positive populations of parental HCT116 and HCT116 FR cells treated with 5-FU for 24 h. b HCT116 and HCT116 FR cell growth over 5 days. c The relative intensities of BODIPY 493/503 staining in HCT116 and HCT116 FR cells treated with iDGAT1/2 for 48 h. The populations of PI-positive cells treated with 10 μM RSL3 and iDGAT1/2 (d) or 10 μM ML162 and iDGAT1/2 (e) for 24 h. The relative numbers of parental T47D (f) and T47D PR (g) cells measured every 3 days. Cells were cultured with or without iCDK4/6. h The relative intensities of BODIPY 493/503 staining in T47D and T47D PR cells treated with iDGAT1/2 for 48 h. The populations of PI-positive cells treated with 100 nM RSL3 and iDGAT1/2 for 24 h (i) or 200 nM ML162 and iDGAT1/2 for 18 h (j). Volumes (k) and weights (l) of HCT116 xenograft tumors in the indicated treatment groups. n = 8 for vehicle, IKE+Lip-1, iDGAT1/2 + IKE+Lip-1; n = 7 for IKE, iDGAT1/2 + IKE; n = 6 for iDGAT. Volumes (m) and weights (n) of HCT116 FR xenograft tumors in the indicated treatments groups, n = 8, except for iDGAT (n = 7). Immunochemical scoring of 4-HNE (o) and PLIN3 (p) staining in tumor sections. n = 8 in HCT116, except for vehicle, IKE (n = 10) and IKE+Lip-1 (n = 9). n = 8 in HCT116 FR, except for IKE (n = 10), iDGAT+IKE (n = 9) and iDGAT+IKE+Lip-1 (n = 6) were used for 4-HNE and n = 8 was used for PLIN3 staining. q Working model. See the Discussion for a detailed description. PLs, phospholipids; LOO^•, peroxy radical; LD, lipid droplet. Figure 4q is created using BioRender. Mean (±SD) values are shown. n = 3. n indicates independent repeats (a–j), P values were calculated using two-way ANOVA (a, b, f, g, k, and m) or an unpaired, two-tailed t-test. n.s. not significant. Source data are provided as a Source Data file.