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. 2024 Jan 2;15:52. doi: 10.1038/s41467-023-44413-6

Fig. 4. CT-SLiM modifications differentially affect membrane remodeling and fission.

Fig. 4

a Representative kymographs showing the remodeling of freely suspended NTs upon addition of 0.5 μM of WT Drp1 or CT variants in the presence of 1 mM GTP. NT membrane fluorescence is displayed in cyan pseudocolor for clarity. Left images correspond to the initial frame of the kymographs. Right image sequences correspond to the framed region of the kymographs. Purple arrows indicate NT fission. b Distributions of the radii of free-standing NTs that underwent fission (yes) or were only constricted (no) upon addition of 0.5 μM WT Drp1 or CT variants in the presence of 1 mM GTP. The numbers on top of each box represent the total number of NTs for each condition. Error bars are SD, n = 3 independent experiments. Box plots indicate median (middle line), 25th and 75th percentile (box) and outliers (whiskers). c Cryo-EM images showing WT Drp1, CT+ Drp1, and ΔCT4 Drp1 assembled on preformed NTs in the presence of 1 mM GTP. White arrowheads indicate Drp1 scaffolds on highly curved NT membranes. Black arrowhead shows curvature-adaptable assembly of WT Drp1 also on relatively flat (low curvature) membrane regions, not observed with CT+ Drp1.