Fig. 3.

MCL suppresses the ferroptosis of macrophage induced by ox-LDL. THP-1 original macrophages are pretreated with MCL (10 μM) for 1 h, then stimulated with 100 μg/ml ox-LDL for 48 h. (A). CCK-8 assay was used to analysis cell viability. N = 5; (B). LDH level in supernatant. N = 5; (C, D). Representative transmission electron microscopy pictures of macrophages and the quantification of relative mitochondrial length. N = 8; (E, F). The lipid ROS level was evaluated by flow cytometry using C11-Bodipy fluorescent probe. N = 5; (G, H) Total artery RNA was extracted from the cells, and mRNA levels of GPX4 and xCT were detected; N = 5. (I, J) Total cell lysates were extracted, and protein levels of GPX4 and xCT were detected; N = 5. (K–N). The levels of MDA, GSH, SOD activity and iron content were measured in macrophages by commercial kits. **p < 0.01, ***p < 0.001 vs CTRL group; #p < 0.05, ###p < 0.001 vs ox-LDL group.