Fig. 5.

MCL enhances NRF2 nuclear translocation in macrophages and ApoE−/− mice. In macrophages, the nuclear lysate was extracted to analysis NRF2 expression. In macrophages and aorta, Nuclear and cytoplasmic lysate were extracted to analysis NRF2 expression. (A, B). Western blot is used to evaluate NRF2 levels. N = 3; (C, D). Confocal fluorescent staining is used to evaluate the nuclear translocation of NRF2. N = 6; (E, F). The protein levels of NRF2 in the aorta nuclear lysates of ApoE^−/− mice. N = 5; (G) The enrichment ability of NRF2 on GPX4 and xCT promoter using ChIP-qPCR; ***p＜0.001, vs control or ND group. ###p < 0.001, vs ox-LDL or HD group.