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. 2023 Dec 7;69:102987. doi: 10.1016/j.redox.2023.102987

Fig. 8.

Fig. 8

MCL promotes the dissociation of KEAP1 and NRF2 (A). THP-1 original macrophages are pretreated with MCL (10 μM) for 1 h, then stimulated with 100 μg/ml ox-LDL for 48 h. (A). Co-immunoprecipitation was used to evaluate the binding of KEAP1 and NRF2. (B, C). The residues detail of the interaction. (D). Venn diagram representing the overlap of the identified sites between NRF2/KEAP1 and MCL/KEAP1. (E). KEAP1-WT and KEAP1-R483S were transfected into macrophages. (F) Co-immunoprecipitation was used to evaluate the binding of KEAP1 and NRF2. N = 3; (G). Immunofluorescence was used to evaluate the binding of KEAP1 and NRF2. N = 3; (H). In macrophages, the nuclear lysate was extracted to analysis NRF2 expression. N = 5; ***p < 0.001. vs control group. ###p < 0.001 vs ox-LDL group.